Silver Diamine Fluoride and Potassium Iodide Disruption of In Vitro Streptococcus mutans Biofilm
Methods: Fifty μl of an overnight S. mutans culture (106 CFU per mL) in Tryptic Soy Broth (TSB) and three ml of fresh TSB supplemented with one percent sucrose (TSBS) were incubated for 24 hours to establish an S. mutans biofilm in six-well tissue culture plates. Four treatments (SDF, SSKI, SDF plus SSKI, and untreated control) were used to disrupt the biofilm. The biofilm groups were each treated with reagent and washed; the biofilm was collected, diluted, and spiral-plated onto blood agar plates; and an automated counting machine was used to determine the bacterial colony forming units (CFU).
Results: The control had significantly more CFU than the SSKI, SDF, and SDF plus SSKI groups (P<.0001). The SSKI group had significantly more CFU than the SDF and SDF plus SSKI groups (P<.0001). The SDF group had significantly fewer CFU than the SDF plus SSKI group (P=.02). The reduction from the control was more than seven-fold for SDF, four-fold for SDF plus SSKI, and two-fold for SSKI.
Conclusions: SDF alone, SDF plus SSKI, and SSKI disrupted an established S. mutans biofilm. SDF alone had the greatest overall disruption.
Document Type: Research Article
Affiliations: 1: Assistant professor and an assistant program director, in the School of Dentistry, at Indiana University, Indianapolis, Ind., USA;, Email: [email protected] 2: Resident, in the School of Dentistry, at Indiana University, Indianapolis, Ind., USA 3: Chair and a professor, Department of Pediatric Dentistry, in the School of Dentistry, at Indiana University, Indianapolis, Ind., USA 4: Biostatistician, Department of Biostatistics, School of Medicine, all at Indiana University, Indianapolis, Ind., USA 5: Associate Dean for Graduate Education and Professor, Department of Biomedical and Applied Sciences, in the School of Dentistry, at Indiana University, Indianapolis, Ind., USA
Publication date: September 1, 2018
- Acquired after the merger between the American Society of Dentistry for Children and the American Academy of Pediatric Dentistry in 2002, the Journal of Dentistry for Children (JDC) is an internationally renowned journal whose publishing dates back to 1934. Published three times a year, JDC promotes the practice, education and research specifically related to the specialty of pediatric dentistry. It covers a wide range of topics related to the clinical care of children, from clinical techniques of daily importance to the practitioner, to studies on child behavior and growth and development. JDC also provides information on the physical, psychological and emotional conditions of children as they relate to and affect their dental health.
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