Skip to main content
padlock icon - secure page this page is secure

Open Access Strain-specific Differences in the Effects of Lymphocytes on the Development of Insulin Resistance and Obesity in Mice

Download Article:
 Download
(PDF 598.3 kb)
 
Obesity is characterized as a chronic, low-grade inflammatory disease owing to the infiltration of the adipose tissue by macrophages. Although the role of macrophages in this process is well established, the role of lymphocytes in the development of obesity and metabolism remains less well defined. In the current study, we fed WT and Rag1–/– male mice, of C57BL/6J and BALB/c backgrounds, high-fat diet (HFD) or normal diet for 15 wk. Compared with WT mice, Rag1–/– mice of either of the examined strains were found less prone to insulin resistance after HFD, had higher metabolic rates, and used lipids more efficiently, as shown by the increased expression of genes related to fatty acid oxidation in epidydimal white adipose tissue. Furthermore, Rag1–/– mice had increased Ucp1 protein expression and associated phenotypic characteristics indicative of beige adipose tissue in subcutaneous white adipose tissue and increased Ucp1 expression in brown adipose tissue. As with inflammatory and other physiologic responses previously reported, the responses of mice to HFD show strain-specific differences, with increased susceptibility of C57BL/6J as compared with BALB/c strain. Our findings unmask a crucial role for lymphocytes in the development of obesity and insulin resistance, in that lymphocytes inhibit efficient dissipation of energy by adipose tissue. These strain-associated differences highlight important metabolic factors that should be accommodated in disease modeling and drug testing.

44 References.

No Supplementary Data.
No Article Media
No Metrics

Document Type: Research Article

Affiliations: 1: Biomedical Research Foundation of the Academy of Athens, Clinical Experimental Surgery & Translational Research, Athens, University of Crete, School of Medicine, Heraklion, Crete, Greece 2: Biomedical Research Foundation of the Academy of Athens, Clinical Experimental Surgery & Translational Research, Athens, Greece 3: Department of Pharmacology, University of Patras Medical School, Patras, Greece 4: Biomedical Research Foundation of the Academy of Athens, Clinical Experimental Surgery & Translational Research, Athens, Greece;, Email: [email protected] 5: Biomedical Research Foundation of the Academy of Athens, Clinical Experimental Surgery & Translational Research, Athens, Greece, Endocrine Division, Boston Children's Hospital, Boston, Massachuttes

Publication date: February 1, 2018

More about this publication?
  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

    Attention Members: To access the full text of the articles, be sure you are logged in to the AALAS website.

    Attention: please note, due to a temporary technical problem, reference linking within the content is not available at this time

  • Editorial Board
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Membership Information
  • Information for Advertisers
  • For issues prior to 1998
  • Institutional Subscription Activation
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more