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Open Access Age-Associated Differences in Hematopoietic Stem and Progenitor Cells of Mice

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Establishing the appropriate yet minimal number of control mice for experiments is a critical step in experimental design. This decision is particularly important regarding the study of the hematopoietic system over time, given various age-associated changes in murine hematopoietic cell populations. Here we used flow cytometry to serially monitor the frequencies of hematopoietic stem cells, common lymphoid progenitor cells, and common myeloid progenitor cells and RT-PCR assays to study the levels of Ly6a (Sca1), Slamf1, Ikzf1, and Cebpa—4 genes that control the hematopoietic process—in wildtype male and female mice with a B6SJL genetic background. These analyses revealed many differences, both at the cellular and mRNA levels, between immature and mature mice at various developmental stages. In conclusion, although it is necessary to minimize the number of mice possible insofar as possible to reduce animal use and meet animal welfare requirements, the numerous differences shown by our findings highlight the need to establish controls for every time point selected for the study of the hematopoietic system cells. This need is especially crucial when comparing immature and mature stages of mouse development.

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Document Type: Research Article

Affiliations: 1: Laboratory of Genetics and Biochemistry (LAGENBIO), Veterinary Faculty of Zaragoza, Aragon Institute of Food Agriculture, University of Zaragoza, Zaragoza, Spain 2: Biochemistry Department, Health Research Institute, Madrid, Spain 3: Laboratory of Genetics and Biochemistry (LAGENBIO), Veterinary Faculty of Zaragoza, Aragon Institute of Food Agriculture, University of Zaragoza, Zaragoza, Spain;, Email: [email protected]

Publication date: February 1, 2017

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  • Comparative Medicine (CM), an international journal of comparative and experimental medicine, is the leading English-language publication in the field and is ranked by the Science Citation Index in the upper third of all scientific journals. The mission of CM is to disseminate high-quality, peer-reviewed information that expands biomedical knowledge and promotes human and animal health through the study of laboratory animal disease, animal models of disease, and basic biologic mechanisms related to disease in people and animals.

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