@article {Saha:2016:0891-3668:S16,
title = "Laboratory Methods for Determining Etiology of Neonatal Infection at Population-based Sites in South Asia",
journal = "The Pediatric Infectious Disease Journal",
parent_itemid = "infobike://wk/inf",
publishercode ="wk",
year = "2016",
volume = "35",
number = "5",
publication date ="2016-05-01T00:00:00",
pages = "S16-S22",
itemtype = "ARTICLE",
issn = "0891-3668",
eissn = "1532-0987",
url = "https://www.ingentaconnect.com/content/wk/inf/2016/00000035/00000005/art00005",
doi = "doi:10.1097/INF.0000000000001101",
keyword = "blood culture, molecular diagnostics, sepsis, neonatal, ANISA, etiology",
author = "Saha, Samir K. and Islam, Mohammad Shahidul and Qureshi, Shahida M. and Hossain, Belal and Islam, Maksuda and Zaidi, Anita K. and Modak, Joyanta K. and Al-Emran, Hasan M. and Diaz, Maureen H. and McGee, Lesley and Winchell, Jonas M.",
abstract = "
Background:
The Aetiology of Neonatal Infection in South Asia (ANISA) study aims to determine the etiology of neonatal infections in 5 population-based sites in Bangladesh, India and Pakistan. 
Methods:
The main laboratory challenges in ANISA were selection
and consistent implementation of laboratory methods at participating sites with varied infrastructure. The other specific challenges included (1) specimen collection and transport to designated study laboratories and timely processing in rural settings; (2) minimal or nonexistent laboratory
facilities at the field sites; (3) obtaining sufficient volumes of blood from enrolled infants aged 059 days and (4) caregivers concerns about collection of clinical specimens from young infants. An additional challenge was selecting an appropriate molecular platform from multiple
available options, all with limited field validation, for use in determining infection in young infants. 
Conclusions:
This article describes how the challenges of specimen collection, transport and processing and implementation of laboratory methods have been addressed
in the ANISA study. It also describes the measures taken to improve detection of microorganisms causing young infant infections by enhancing the sensitivity of existing laboratory methods for pathogen detection.",
}