Skip to main content
padlock icon - secure page this page is secure

Open Access Advancements in Molecular Techniques and Applications in Environmental Engineering

Download Article:
(PDF 218.6 kb)
Molecular biological tools continue to be well suited to the challenges and complexity of research focused on the natural and engineered environments. These methods are being applied to an increasing range of efforts in environmental engineering research and practice for an improved understanding of important biological processes. While real-time quantitative PCR (qPCR) has become a staple analytical technique in the molecular toolbox, other nucleic acid-based techniques are gaining popularity. Microbial community analysis has seen an increasing impact in the literature in part due to the diverse array of analytical techniques that are able to provide information on structural changes including catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), microarrays, denaturing gradient gel electrophoresis (DGGE), terminal restriction fragment length polymorphism (T-RFLP) and next generation sequencing technologies. Novel biosensors that integrate molecular analysis are being developed to rapidly detect and quantify microorganisms, estrogenic compounds and other targets of interest. The advent of advanced molecular analyses has resulted in an exponential rise in genomic, transcriptomic, and metabolomic data. Hence, methods involving quality control are gaining popularity as the concern for rigorous standards in molecular data collection and analysis keeps pace with demand. This review will provide environmental engineers and microbiologists an overview of important advancements in molecular techniques and highlight the application of these methods in diverse environments.
No References for this article.
No Supplementary Data.
No Article Media
No Metrics


Document Type: Research Article

Publication date: 01 October 2012

More about this publication?
  • Water Environment Research (WER) is published monthly, including an annual Literature Review. A subscription to WER includes access to the latest content back to 1992, as well as access to fast track articles. An individual subscription is valid for 12 months from month of purchase.

    Water Environment Research (WER) publishes peer-reviewed research papers, research notes, state-of-the-art and critical reviews on original, fundamental and applied research in all scientific and technical areas related to water quality, pollution control, and management. An annual Literature Review provides a review of published books and articles on water quality topics from the previous year.

    Published as: Sewage Works Journal, 1928 - 1949; Sewage and Industrial Wastes, 1950 - 1959; Journal Water Pollution Control Federation, 1959 - Oct 1989; Research Journal Water Pollution Control Federation, Nov 1989 - 1991; Water Environment Research, 1992 - present.
  • Editorial Board
  • Information for Authors
  • Submit a Paper
  • Subscribe to this Title
  • Membership Information
  • Information for Advertisers
  • WEF Bookstore
  • Ingenta Connect is not responsible for the content or availability of external websites
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more