Monoclonal-based enzyme-linked immunosorbent assay for the detection of zearalenone in cereals
A monoclonal antibody against zearalenone (ZEA) was produced and used successfully to develop a direct competitive enzyme-linked immunosorbent assay (DC-ELISA) for the analysis of ZEA in cereals. This DC-ELISA had a limit of detection of 0.15 ± 0.02 µg l-1 and an IC50 value of 1.13 ± 0.16 µg l-1. Matrix interference was minimized by dilution of the sample extract before ELISA assays. Aqueous methanol (80%) gave good extraction efficiencies, and the recovery from spiked rice, barley, and corn samples averaged between 87 and 112%. Although ZEA was detected in seven (9%) of 80 rice samples and in eight (16%) of 50 barley samples, the concentration of ZEA in samples was around or below the limit of detection of DC-ELISA. Among 38 corn samples, ZEA was detected in nine (24%) samples in the range 41.0-909.8 µg kg-1. Re-analysis of the ELISA-positive corn samples by high-performance liquid chromatography (HPLC) confirmed that seven (18%) corn samples were positive. The ZEA results for corn showed very good agreement between DC-ELISA and a commercial AgraQant® zearalenone kit (r2 = 0.98). Thus, the monoclonal antibody-based DC-ELISA could be applied to the preliminary screening of ZEA contamination when analysis of a large sample number is needed.
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enzyme-linked immunosorbent assay (ELISA);
Document Type: Research Article
Division of Applied Life Science (Brain Korea 21 Program), Korea
Division of Applied Life Science (Brain Korea 21 Program), Korea,Mechnikov Research Institute of Vaccine and Sera of Russian Academy of Medical Sciences, Moscow, Russia
Division of Applied Life Science (Brain Korea 21 Program), Korea,Chemistry Faculty, Division of Chemical Enzymology, M. V. Lomonosov Moscow State University, Moscow, Russia
Chemistry Faculty, Division of Chemical Enzymology, M. V. Lomonosov Moscow State University, Moscow, Russia
August 1, 2008
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