Three hundred and forty-nine breakfast and infant cereal samples were collected at retail level across Canada from 2002 to 2005. They included rice-, soy-, barley-based and mixed-grain infant cereals, corn-, wheat-, rice-based and mixed-grain breakfast cereals, and were analysed for aflatoxins B 1 , B 2 , G 1 and G 2 using a modified AOAC International official method. An immunoaffinity column was used for the cleanup and purification of extracts. Determination of aflatoxins was by LC using post-column derivatization with pyridinium hydrobromide perbromide and fluorescence detection. Results indicated that 50% of both breakfast and infant cereals had detectable levels (limit of detection=0.002ngg −1 ) of aflatoxin B 1 , which is the most toxic of the four toxins. The levels found varied from 0.002 to 1.00ngg −1 for aflatoxin B 1 , from 0.002 to 0.14ngg −1 for aflatoxin B 2 , from 0.008 to 0.27ngg −1 for aflatoxin G 1 , and from 0.008 to 0.048ng g −1 for aflatoxin G 2 . Only 4% of the breakfast cereals and 1% of the infant cereals had aflatoxin B 1 levels exceeding 0.1ngg −1 , which is the European Union maximum limit for aflatoxin B 1 in baby foods and processed cereal-based foods for infants and young children.
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Aflatoxin B 1;
Document Type: Research Article
Food Laboratories Division, Health Products and Food Branch, Health Canada, 2301 Midland Avenue, Scarborough, Ontario M1P 4R7, Canada
Food Research Division, Health Products and Food Branch, Health Canada, Address Locator 2203D, Ottawa, Ontario, K1A 0L2, Canada
July 1, 2006
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