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Presence and metabolism of the anabolic steroid boldenone in various animal species: a review

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The review summarizes current knowledge on the possible illegal use of the anabolic steroid boldenone. The presence of boldenone and metabolites in different animal species and the possibility of the occurrence of endogenous boldenone and metabolites is assessed, as are the methods of analysis used for detection. Different laboratories in the European Union have examined the occurrence of boldenone and its metabolites. The results were discussed at different meetings of a European Commission DG-SANCO Working Party and summarized in an expert report. The situation of the different laboratories at this time is also covered herein. The overall conclusion of the Working Party was that there was a necessity for further research to distinguish between naturally occurring and illegally used boldenone forms. The confirmation of the presence of boldenone metabolites (free and conjugated forms) in certain matrices of animals is proposed as a marker for the illegal treatment with boldenone.
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Keywords: anabolic steroids; boldenone; bovine; calves; endogenous; illegal treatment; metabolism; phytosterol

Document Type: Research Article

Affiliations: 1: LCA (Laboratory of Chemical Analysis) Department of Veterinary Public Health and Food Safety Faculty of Veterinary Medicine Ghent University Salisburylaan 133 B-9820 Merelbeke Belgium 2: TNO Nutrition and Food Research Department of Residue Analysis Product Group Hormones and Veterinary Drugs PO Box 360 NL-3700 AJ Zeist the Netherlands 3: RIVM (European Union Community Reference Laboratory for Residues) A. Van Leeuwenhoeklaan 9 PO Box 1 NL-3720 BA Bilthoven the Netherlands 4: LABERCA--Ecole Nationale Vétérinaire de Nantes Route de Gachet BP 50707 F-44307 Nantes Cedex 3 France 5: Laboratory of Veterinary Medicine Section of Anabolic Residues in Foods of Animal Origin National Institute of Health Viale Regina Elena 299 Rome Italy 6: Federal Food Laboratory Braemkasteelstraat 59 B-9050 Gentbrugge Belgium

Publication date: June 1, 2004

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