Flow-through spectrophotometric sensor for the determination of saccharin in low-calorie products
A simple, rapid and inexpensive monoparameter flow-through sensor has been developed for the determination of saccharin in low calorie and dietary products. The method is based on the transient adsorption of the sweetener on Sephadex G-25 solid phase packed to a height of 20 mm in the flow cell. The optimal transient retention of the synthetic sweetener, in terms of sensitivity and sampling frequency, was obtained when pH 2.75 citric acid-sodium citrate buffer 5 × 10−3 M was used as a carrier at a flow-rate of 1.5 ml min−1. Saccharin was determined measuring its intrinsic absorbance at 217 nm at its residence time. Calibration graphs for peak height and peak area were linear over the range 5.0-200.0 g ml−1, RSD 1.18%, and 1.0-200.0 g ml−1, RSD 0.78%, respectively. Saccharin was determined in several food samples measuring height or area peak, obtaining recoveries ranging between 98-104 and 99-102% for height and area peak, respectively. The procedure was validated for use in the determination of saccharin in low calorie and dietary products giving reproducible and accurate results.
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Document Type: Research Article
Affiliations: Department of Analytical Chemistry Faculty of Science University of Granada E-18071 Granada Spain
Publication date: January 1, 2004