Skip to main content
padlock icon - secure page this page is secure

Simultaneous determination of 4-hydroxy-2-alkenals, lipid peroxidation toxic products

Buy Article:

$63.00 + tax (Refund Policy)

Lipid peroxidation has been associated with cell injury and possible carcinogenecity, for the products can damage cellular membrane and DNA. Among the products formed from lipid peroxidation, 4-hydroxy-2-alkenals are the major components and have been suggested to play a role in cellular toxicity. However, the difficulties of the quantification due to their strong reactivity during laboratory procedures hinder the exposure measurement of the compounds. A novel detection method based on the conversion of 4-hydroxy-2-alkenals to stable diols and to subsequent ortho-esters has been developed. Simultaneous detection in a sensitive single-ion monitoring mode by GC/MS was carried out, monitoring m/z 129, which was the common fragment ion for the ortho-esters derived from 4-hydroxy-2-alkenals. The proposed method was used to quantify 4-hydroxy-2-nonenal in oils and in oxidized arachidonic acid.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: 4-hydroxy-2-alkenals; HNE; HOE; lipid peroxidation; simultaneous detection

Document Type: Research Article

Publication date: April 1, 2003

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more