A survey for aflatoxin B1 (AFB1), fumonisin B1 and ochratoxin A (OTA) was conducted on 127 samples that included 30 food-grade barley, 32 barley foods, 18 food-grade corn and 47 corn foods, randomly collected during 1998–99 in Seoul, Korea. The presence of mycotoxins was analysed by direct competitive enzyme-linked immunosorbent assay (ELISA), and most of the positive samples from ELISA were confirmed using high-performance liquid chromatography (HPLC). Recoveries of AFB1 and OTA spiked at 10 ng g–1 and FB1 spiked at 50 ng g–1 were 106, 87 and 105% by ELISA, whereas those by HPLC were 80, 79 and 84%, respectively. Detection limits by ELISA for AFB1, FB1 and OTA were 1, 5 and 5 ng g–1, and those by HPLC were 0.6, 35 and 1 ng g–1. Naturally occurring AFB1, FB1 and OTA were found in 4/32 (12%), 2/32(6%) and 4/32 (12%) samples of barley foods with an average of 26, 16 and 9 ng g–1, respectively. AFB1 and FB1 in corn foods were detected in 4/47 (8%) and 9/47 (19%) samples with the average being 20 and 74 ng g–1, while no OTA was found in any corn foods samples. No AFB1, FB1 or OTA was detected in any of food-grade barley and corn samples. This is the first report on the natural co-occurrence of AFB1 and FB1 in barley and corn foods as well as on surveillance of OTA in Korea.
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AFLATOXIN B1 (AFB1);
BARLEY AND CORN FOODS;
ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA);
FUMONISIN B1 (FB1);
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC);
OCHRATOXIN A (OTA)
Document Type: Research Article
The Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea.
Korea Food Research Institute, Seongnam, Kyunggi 463-420, Korea
The Institute of Biotechnology, Korea University, 5-1, Anam-dong, Sungbuk-gu, Seoul 136-701, Korea.
November 1, 2002
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