Aflatoxin B1 (AFB1) was found in 35 of 60 (58.3%) meju samples with an average concentration of 7.3ng/g by ELISA. Contamination of AFB1 was confirmed in 25 of 60 samples (41.6%) using HPLC, with an average concentration of 6.9ng/g. Mean recoveries from meju ranged from 107% to 170% for AFB1 using ELISA at a spiking range of 1 to 50ng/g. Over the same range, recoveries using HPLC were from 70% to 83%. The levels of AFB1 determined by ELISA and by HPLC demonstrated a close relationship between the two methods (r2 = 0.9324) employed in this study. In order to evaluate the potential health risks of AFB1 on Koreans consuming meju, we calculated the estimated probable daily intake (PDI) based on the average contamination levels and compared it with the estimated tolerable daily intake (TDI). The PDIs of AFB1 from kanjang and dwenjang were determined to be 0.04 and 0.21ng/kg bw/day, respectively, and were higher than TDIs.
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ENZYME-LINKED IMMUNOSORBENT ASSAY;
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY;
Document Type: Research Article
The Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea
Korea Food Research Institute, Seongnam, Kyunggi 463-420, Korea
Department of Food Science and Technology, Chung-Ang University, Ansung 456-756, Korea
February 1, 2001
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