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Use of an immunoassay as a means to detect polychlorinated biphenyls in animal fat

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In this paper we describe the validation and utilization of the Hybrizyme TM DELFIA immunoassay for detecting polychlorinated biphenyls (PCBs) in animal fat. The immunoassay incorporates a rapid sample processing protocol that has been optimized to detect concentrations of PCBs in animal fat at or above 200ng/g. The limit of detection of the assay was less than 50ng/g. When the action level for the DELFIA assay was set at 100ng/g to detect samples having 200ng/g or more PCBs, the false negative rate was estimated at 0.2% . The false positive rate depends on the concentration of PCBs in the sample population and was estimated at 4% and 7% for samples having 10ng/ g and 20ng/g PCBs, respectively. By utilizing the DELFIA assay system to select for positive samples and GC/MS analysis for confirmation analysis, an accurate and cost-effective programme has been established for the high throughput detection of PCBs in animal products.
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Keywords: ANIMAL FAT; DELFIA; IMMUNOASSAY; POLYCHLORINATED BIPHENYLS; SCREENING METHOD

Document Type: Research Article

Affiliations: 1: European Commission, DG Joint Research Centre, Institute for Health and Consumer Protection, Food Products and Consumer Goods Unit; TP 260, I-21020 Ispra, Italy 2: Hybrizyme, Raleigh, North Carolina 27607, USA

Publication date: February 1, 2001

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