A survey of zearalenone in corn using Romer Mycosep 224 column and high performance liquid chromatography
A survey of zearalenone (ZEA) in corn from various regions of Brazil was carried out by the analysis of 380 corn samples, of which 30 samples (7.8%) were found to be contaminated in the range of 46.7-719 mu g/kg. ZEA was extracted with acetonitrile-water (84:16, v/v), cleaned-up on a Romer MycosepTM 224 column, separated, detected and quantified by high performance liquid chromatography (HPLC). The in-house method characteristics of linearity, accuracy, precision, and detection limit were defined by means of recovery tests with spiked corn samples in the range of 35.8-716 g/kg and the analysis of a naturally-contaminated sample (n=7). The mean recovery for ZEA was 99.4% and the relative standard deviation (RSD) varied from 0.7 to 26.6% in the range studied. The method has been shown to be accurate, quick and reliable for determination of zearalenone in corn.
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Document Type: Research Article
Affiliations: Ministry of Agriculture and Supply
Publication date: January 1, 2001