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Isolation of Imperatorin, Oxypeucedanin, and Isoimperatorin from Angelica dahurica (Fisch. ex Hoffm) Benth. et Hook by Stepwise Flow Rate High‐Speed Countercurrent Chromatography

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Stepwise flow rate preparative high‐speed countercurrent chromatography (HSCCC) was successfully used for isolation and purification of imperatorin, oxypeucedanin, and isoimperatorin from a crude root extract of A ngelica dahurica (Fisch. ex Hoffm) Benth. et Hook. The separation was performed with a two‐phase solvent system composed of n‐hexane‐ethyl acetate‐methanol‐water at volume ratio of 5:5:4:6, v/v/v/v, which had been selected by analytical HSCCC. About a 600 mg amount of the crude extract was separated in a one step operation, yielding 35.6 mg of imperatorin, 16.4 mg of oxypeucedanin, and 22.7 mg of isoimperatorin, all at a high purity of over 98%.
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Keywords: Angelica dahurica; High‐speed countercurrent chromatography; Imperatorin; Isoimperatorin; Oxypeucedanin; Stepwise flow

Document Type: Research Article

Affiliations: 1: Applied Chemistry Department, Faculty of Sciences, Beijing University of Chemical Technology, Beijing, P. R. China 2: Center for Biochemistry and Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA

Publication date: 01 January 2006

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