Skip to main content
padlock icon - secure page this page is secure

Isolation of Imperatorin, Oxypeucedanin, and Isoimperatorin from Angelica dahurica (Fisch. ex Hoffm) Benth. et Hook by Stepwise Flow Rate High‐Speed Countercurrent Chromatography

Buy Article:

$55.00 + tax (Refund Policy)

Stepwise flow rate preparative high‐speed countercurrent chromatography (HSCCC) was successfully used for isolation and purification of imperatorin, oxypeucedanin, and isoimperatorin from a crude root extract of A ngelica dahurica (Fisch. ex Hoffm) Benth. et Hook. The separation was performed with a two‐phase solvent system composed of n‐hexane‐ethyl acetate‐methanol‐water at volume ratio of 5:5:4:6, v/v/v/v, which had been selected by analytical HSCCC. About a 600 mg amount of the crude extract was separated in a one step operation, yielding 35.6 mg of imperatorin, 16.4 mg of oxypeucedanin, and 22.7 mg of isoimperatorin, all at a high purity of over 98%.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: Angelica dahurica; High‐speed countercurrent chromatography; Imperatorin; Isoimperatorin; Oxypeucedanin; Stepwise flow

Document Type: Research Article

Affiliations: 1: Applied Chemistry Department, Faculty of Sciences, Beijing University of Chemical Technology, Beijing, P. R. China 2: Center for Biochemistry and Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA

Publication date: 01 January 2006

  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more