Stepwise flow rate preparative high‐speed countercurrent chromatography (HSCCC) was successfully used for isolation and purification of imperatorin, oxypeucedanin, and isoimperatorin from a crude root extract of A ngelica dahurica (Fisch. ex Hoffm) Benth. et Hook. The separation was performed with a two‐phase solvent system composed of n‐hexane‐ethyl acetate‐methanol‐water at volume ratio of 5:5:4:6, v/v/v/v, which had been selected by analytical HSCCC. About a 600 mg amount of the crude extract was separated in a one step operation, yielding 35.6 mg of imperatorin, 16.4 mg of oxypeucedanin, and 22.7 mg of isoimperatorin, all at a high purity of over 98%.
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High‐speed countercurrent chromatography;
Document Type: Research Article
Applied Chemistry Department, Faculty of Sciences, Beijing University of Chemical Technology, Beijing, P. R. China
Center for Biochemistry and Biophysics, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA
Publication date: 01 January 2006