Dispersive liquid–liquid microextraction with solidification of floating organic drop combined with high performance liquid chromatography for the determination of phenolic oestrogens from human urine and water samples
A novel, simple and rapid method, termed dispersive liquid–liquid microextraction with solidification of floating organic drop coupled to high performance liquid chromatography, was developed for analysis of three phenolic oestrogens including diethylstilbestrol, dienestrol and
hexestrol in human urine and water samples. The parameters of dispersive liquid–liquid microextraction with solidification of floating organic drop procedure including sample pH, type and volume of disperser solvent, and type and volume of extraction solvent were optimised. High performance
liquid chromatography was applied for the phenolic oestrogens’ analysis. Under the optimum extraction and detection conditions, excellent analytical performances were attained. Good linear relationships (r ≥ 0.998) between peak area and concentration for diethylstilbestrol
and dienestrol were optimised from 0.1 to 20 µg/mL, for hexestrol from 2 to 50 µg/mL. Method detection limits of 28.6–666.7 ng/mL were achieved. Satisfactory relative recoveries ranging from 72% to 122% were determined for urine, lake and tap water samples,
with relative standard deviations (RSDs, n = 6) of 1.5–9.8%. The developed dispersive liquid–liquid microextraction with solidification of floating organic drop-high performance liquid chromatography method has a great potential in routine residual analysis of trace phenolic
oestrogens in biological and water samples.
Keywords: Dispersive liquid–liquid microextraction with solidification of floating organic drop; high performance liquid chromatography; phenolic oestrogens; urine sample; water sample
Document Type: Research Article
Affiliations: 1: Laboratory of Pathogenic Biology and Immunology, School of Basic Medical Science, Hainan Medical University, Haikou, China 2: Department of Environmental Science, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou, China 3: Institute of Environment and Plant Protection, Chinese Academy of Tropical Agricultural Sciences, Haikou, China
Publication date: 09 April 2019
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