Skip to main content
padlock icon - secure page this page is secure

Screening fluorescent method for the fluoroquinolone family in groundwater samples from intensive livestock production systems

Buy Article:

$60.00 + tax (Refund Policy)

A fast and simple screening fluorescent method was developed and applied for detection of quinolones in groundwater samples. The experimental conditions for quinolone family detection in 96-well plates were acid media (pH = 4.85) from acetate buffer solution (0.1 mol L−1) and 5.8 × 10−3 mol L−1 of anionic surfactant sodium dodecyl sulfate (SDS) as micellar media at excitation and emission wavelengths of 280 and 450 nm, respectively. The developed method was validated to guarantee the quality of the results reported. Thus, the decision limit (CCα) of ciprofloxacin of 6.8 μg L−1, the most prescript quinolone in our country, was selected as the cut-off level to classify the water samples as ‘suspect’ or ‘negative’ referred to quinolone content. The method showed good recoveries ranging between 80 and 114% for 6.8 μg L−1 ciprofloxacin with relative standard deviation values lower than 13%. Moreover, other families of antibiotics such as aminoglycoside, penicillin, macrolide, sulfonamide and tetracycline did not present interference in the quinolone detection. Groundwater samples from Argentine regions with intensive livestock activities were analysed by this method, and the results had a good correlation with a reference method based on ultraperformance liquid chromatography coupled to mass spectrometry.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: Screening method; emerging contaminant; fluorometry; fluoroquinolone; livestock groundwater

Document Type: Research Article

Affiliations: 1: Laboratorio de Sensores y Biosensores, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina 2: Cátedra de Biofísica, Departamento de Ciencias Básicas, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Esperanza, Argentina

Publication date: September 2, 2018

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more