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Acetylcholinesterase immobilised eggshell membrane-based optical biosensor for organophosphate detection

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The development of an optical biosensor for the determination of malathion based on acetylcholinesterase (AChE) inhibition using Ellman’s reagent is reported. The AChE has been immobilised onto the eggshell membrane (ESM) using glutaraldehyde as a cross-linking agent. Scanning Electron Microscopic (SEM) studies and Fourier Transformed Infra-Red (FTIR) characterisations have been carried out to affirm the successful immobilisation of AChE onto the ESM. Under optimum conditions, the developed biosensor estimated the pesticide concentration in the range of 0.1–50 ng/mL with a limit of detection (LOD) of 0.1 ng/mL within 30 min. Parameters affecting the biosensor response such as concentration of enzyme, substrate and inhibition time were optimised. The stability and reusability of the AChE/ESM sensor have been observed as 31 days at 4°C and two times, respectively.
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Keywords: Ellman’s reagent; acetylcholinesterase; eggshell membrane; glutaraldehyde; optical biosensor

Document Type: Research Article

Affiliations: Department of Biochemistry, Panjab University, Chandigarh, 160014, India

Publication date: September 26, 2015

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