Contaminant detection in treated water using Optiqua’s MiniLab™ biosensing system: a case study for Bisphenol A
An inhibition assay for detection of Bisphenol A (BPA) in treated water has been developed and validated for the MiniLab™ system from Optiqua Technologies. This biosensor-based analytical system can be used for detection of specific contaminants in solution. The system uses an
integrated optic Mach-Zehnder interferometer chip that is functionalised with a biochemical interface layer. The sensor chip surface was first coated with aminodextran which formed a hydrophilic layer suitable for further modification. 4,4-bis(4-hydroxyphenyl) valeric acid (BVA), which is
a structural analogue of BPA, was then successfully coupled to the aminodextran layer. This surface chemistry was used in a qualitative inhibition assay format for the selective detection of the presence of BPA. The assay was developed and optimised using a polyclonal anti-BPA antibody. Samples
from 12 different water matrices of Singapore were spiked with known amounts of BPA and tested with the MiniLab™ system. The working range of the BPA inhibition assay is from 0.5 to 5 µg/L. Intra- and inter-assay variations were measured, with calculated relative standard
deviation averaging of about 15%. BPA concentrations of the spiked samples were also verified and confirmed with LC-MS. Such functionalised biosensor chip can be reused for more than 200 sample injections over a period longer than 6 months. Developed as a robust, user-friendly and cost-effective
system that can be field deployed, the MiniLab™ system enables target-specific assays currently only available in the lab to become available in the field.
Keywords: Bisphenol A; biosensor; inhibition assay; optical immunosensor; water quality
Document Type: Research Article
Affiliations: 1: Optiqua Technologies Pte. Ltd, Singapore, 608576, Republic of Singapore 2: Optisense B.V, Enschede, 7521PA, The Netherlands 3: Water Quality Office, the Public Utilities Board (PUB), Singapore, 608576, Republic of Singapore
Publication date: 16 March 2015
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