Skip to main content
padlock icon - secure page this page is secure

Simultaneous determination of eight antibiotics from distinct classes in surface and wastewater samples by solid-phase extraction and high-performance liquid chromatography–electrospray ionisation mass spectrometry

Buy Article:

$61.00 + tax (Refund Policy)

A reliable multiclass method has been developed and validated for the determination of eight antibiotics from distinct classes (sulfonamides, macrolides, fluoroquinolones, tetracyclines, cephalosporins and dihydrofolate reductase inhibitors) in wastewater – influent and effluent – and surface water from Porto Alegre, Brazil. The pre-concentration and clean-up was conducted with a simple and fast protocol using solid-phase extraction allowing a 100-fold concentration factor. The proposed method was validated by using spiked blank wastewater samples in terms of linearity, repeatability, reproducibility, recovery, matrix effects and limits of detection and quantification. Recovery was obtained in the range of 66–149%. Method limit of quantification ranged between 1.6 and 61.7 ng L−1. Samples (n = 16) were taken from January to August 2011 in one wastewater treatment plant, which uses conventional biological treatment. Sulfamethoxazole and trimethoprim show higher concentration, ranging from >10 to <6500 ng L−1, whereas erythromycin presented the lower amount. Differences between influent and effluent profiles were discussed. Surface water samples (n = 8) were collected in Arroio Diluvio, in four sampling points, in February 2012. From the eight antibiotics analysed, five were detected: sulfamethoxazole, trimethoprim, azythromicyn, ciprofloxacin and norfloxacin, in a concentration range of 376–572 ng L−1, 27–94 ng L−1, 24–40 ng L−1, 16–66ng L−1 and 30–54 ng L−1, respectively.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: SPE, LC-MS/MS; antibiotics; surface water; wastewater

Document Type: Research Article

Affiliations: 1: Laboratório Nacional Agropecuário – LANAGRO/RS, Ministério da Agricultura, Pecuária e Abastecimento, Porto Alegre, Brazil 2: Instituto de Química, Universidade Federal do Rio Grande do Sul – UFRGS, Porto Alegre, Brazil

Publication date: August 9, 2014

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more