Skip to main content
padlock icon - secure page this page is secure

Chemiluminescent enzyme-linked immunosorbent assay on a strip to detect Escherichia coli O157:H7

Buy Article:

$61.00 + tax (Refund Policy)

A fast and sensitive chemiluminescent enzyme-linked immunosorbent assay method to measure pathogenic bacteria, Escherichia coli O157:H7, on immuno-chromatographic membrane was studied. Non-specific binding of proteins on membrane strip was controlled to attain the best performance of immunosensor by optimising the composition of a running buffer. The specificity of the proposed immunostrip was confirmed by conducting experiments for four different micro-organisms. A chemiluminescent signal could be successfully generated from a proposed immunostrip sensing system, and a significant change in the chemiluminescent light intensity with the concentration of target microbes was obtained. E. coli O157:H7 could be quantitatively measured in the range of 1.1 × 103 –1.1 × 107 CFU (colony forming units) mL−1 within 16 min by using the developed chemiluminescent immunostrip.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: Escherichia coli O157:H7; chemiluminescence; chromatographic membrane strip; enzyme-linked immunosorbent assay (ELISA); immunosensor

Document Type: Research Article

Affiliations: 1: Division of Chemical Engineering,Hankyong National University, Anseong, Kyonggi-Do 456-749, Korea 2: Department of NanoBiotechnology, Kyungwon UniversitySeongnam,Kyonggi-Do 461-701, Korea

Publication date: May 15, 2012

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more