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Chemiluminescent enzyme-linked immunosorbent assay on a strip to detect Escherichia coli O157:H7

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A fast and sensitive chemiluminescent enzyme-linked immunosorbent assay method to measure pathogenic bacteria, Escherichia coli O157:H7, on immuno-chromatographic membrane was studied. Non-specific binding of proteins on membrane strip was controlled to attain the best performance of immunosensor by optimising the composition of a running buffer. The specificity of the proposed immunostrip was confirmed by conducting experiments for four different micro-organisms. A chemiluminescent signal could be successfully generated from a proposed immunostrip sensing system, and a significant change in the chemiluminescent light intensity with the concentration of target microbes was obtained. E. coli O157:H7 could be quantitatively measured in the range of 1.1 × 103 –1.1 × 107 CFU (colony forming units) mL−1 within 16 min by using the developed chemiluminescent immunostrip.
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Keywords: Escherichia coli O157:H7; chemiluminescence; chromatographic membrane strip; enzyme-linked immunosorbent assay (ELISA); immunosensor

Document Type: Research Article

Affiliations: 1: Division of Chemical Engineering,Hankyong National University, Anseong, Kyonggi-Do 456-749, Korea 2: Department of NanoBiotechnology, Kyungwon UniversitySeongnam,Kyonggi-Do 461-701, Korea

Publication date: May 15, 2012

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