A novel method for the simultaneous determination of α-naphthol (α-NAP), -naphthol (-NAP) and 1-hydroxypyrene (1-OHP) in human urine has been established by using synchronous fluorescence spectrometry. The measurement was carried out in sodium acetate-boroborax buffer solution (pH = 5.0) with -cyclodextrin (-CD) enhancing fluorescence. At Δ = 23 nm, 1-OHP and -NAP exhibit maximum signal with minimum interferences from α-NAP. At Δ = 175 nm, the signal of α-NAP is not influenced by the presence of 1-OHP and -NAP. The signals detected at these three wavelengths, 360.2 nm, 330.6 nm and 296.4 nm, vary linearly when the concentration of 1-OHP, -NAP and α-NAP is in the range of 0.65-218.3 ng mL-1, 2.8-1441.0 ng mL-1 and 3.6-1586.0 ng mL-1, respectively. The limits of detection (LOD) for α-NAP, -NAP and 1-OHP were 1.53 ng mL-1, 0.78 ng mL-1 and 0.020 ng mL-1 with relative standard deviations (RSD) of 2.3%, 2.4% and 1.8%, respectively. The proposed method was successfully applied for the simultaneous determination of α-NAP, -NAP and 1-OHP in human urine samples, and the obtained results were in good agreement with those obtained by the method of high-performance liquid chromatography (HPLC).
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Document Type: Research Article
College of Public Health, University of South China, Hengyang 421001, China,Hengyand Blood Centre, Hengyang, 421001, China
College of Public Health, University of South China, Hengyang 421001, China
College of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China
January 1, 2011
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