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Simultaneous determination of α-naphthol, -naphthol and 1-hydroxypyrene in urine by synchronous fluorescence spectrometry using -cyclodextrin as a sensitiser

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A novel method for the simultaneous determination of α-naphthol (α-NAP), -naphthol (-NAP) and 1-hydroxypyrene (1-OHP) in human urine has been established by using synchronous fluorescence spectrometry. The measurement was carried out in sodium acetate-boroborax buffer solution (pH = 5.0) with -cyclodextrin (-CD) enhancing fluorescence. At Δ = 23 nm, 1-OHP and -NAP exhibit maximum signal with minimum interferences from α-NAP. At Δ = 175 nm, the signal of α-NAP is not influenced by the presence of 1-OHP and -NAP. The signals detected at these three wavelengths, 360.2 nm, 330.6 nm and 296.4 nm, vary linearly when the concentration of 1-OHP, -NAP and α-NAP is in the range of 0.65-218.3 ng mL-1, 2.8-1441.0 ng mL-1 and 3.6-1586.0 ng mL-1, respectively. The limits of detection (LOD) for α-NAP, -NAP and 1-OHP were 1.53 ng mL-1, 0.78 ng mL-1 and 0.020 ng mL-1 with relative standard deviations (RSD) of 2.3%, 2.4% and 1.8%, respectively. The proposed method was successfully applied for the simultaneous determination of α-NAP, -NAP and 1-OHP in human urine samples, and the obtained results were in good agreement with those obtained by the method of high-performance liquid chromatography (HPLC).
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Keywords: 1-hydroxypyrene; double scans; synchronous fluorescence; urine; α-naphthol; -cyclodextrin; -naphthol

Document Type: Research Article

Affiliations: 1: College of Public Health, University of South China, Hengyang 421001, China,Hengyand Blood Centre, Hengyang, 421001, China 2: College of Public Health, University of South China, Hengyang 421001, China 3: College of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China

Publication date: January 1, 2011

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