Determination of phosphate in freshwater samples by flow-injection with lucigenin chemiluminescence
Lucigenin chemiluminescence (CL) in conjunction with flow-injection analysis (FIA) is used for the determination of phosphate in freshwater samples. The procedure is based on the formation of molybdophosphoric heteropoly acid (MoP-HPA) by the reaction of phosphate and ammonium molybdate
under acidic conditions. CL emission was observed as a result of oxidation of lucigenin in aqueous sodium hydroxide solution in the presence of MoP-HPA. Calibration was linear up to 500 µg L-1 (r2 = 0.9998; n = 8), with a detection limit (S/N = 3) of 0.95 µg L-1. An injection throughput
of 120 h-1, and relative standard deviation (RSD; n = 4) of 1.3-3.2% were achieved in the concentration range studied. An on-line chelating column was used to remove interfering cations. The method was applied to freshwater samples, and the results (51 ± 1.0 - 107 ± 2.0
µg L-1) did not differ significantly from results obtained using a spectrophotometric method (52.5 ± 1.0 - 102 ± 2.0 µg L-1) at 95% confidence level (t-test).
Keywords: chemiluminescence; flow-injection analysis; freshwater samples; lucigenin; molybdate; phosphate
Document Type: Research Article
Affiliations: 1: Department of Chemistry, University of Balochistan, Quetta, Pakistan 2: Department of Environmental Management and Policy, Balochistan University of Information Technology, Engineering and Management Sciences, Quetta, Pakistan
Publication date: 01 October 2010
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