Two series of haptens including 3-phenoxybenzoic acid (PBA) and 3-(2-chloro-3, 3,3-trifluoroprop-1-enyl)-2,2-dimethylcyclo-propanecarboxylic acid (CF3MPA) were used to prepare immunogens through attachment of 4-C or 6-C handles. Class selective antibodies were produced by immunising rabbits. Ab502 showed the highest reactivity towards tau-fluvalinate (IC50 1.3 ng mL-1), -cyhalothrin (IC50 2.3 ng mL-1), cyfluthrin (IC50 2.2 ng mL-1) and fenpropathrin (IC50 18.5 ng mL-1) among the antibodies in a competitive ELISA. The effects of methanol, pH and salt concentration were optimised for maximum efficiency of the ELISA (Enzyme-Linked ImmunoSorbent Assay). Ab502 (1:80000)/2-OVA-1(0.2 µg mL-1) was chosen for ELISA optimisation. Finally, 0.05 M phosphate buffered saline (PBS) at pH 6.5 containing 30% methanol (v/v) was used to dilute the standards. Target analytes in honey samples were extracted with ethyl acetate by sonication. The samples were spiked with three different concentrations of each compound (tau-fluvalinate, 0.5 ng g-1, 3 ng g-1, 12 ng g-1; -cyhalothrin and cyfluthrin 1 ng g-1, 5 ng g-1, 65 ng g-1). The recoveries were 36-59% at the lowest spiking concentration and 61-81% at the higher concentration. This assay might be useful to screen pyrethroid residues in honey or other matrix.
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class selective antibody;
Document Type: Research Article
National Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China
Department of Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, Beijing 100094, China
School of Food Science and Technology, Southern Yangtze University, Wuxi, JiangSu 214122, China
January 1, 2009
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