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Determination of carbofuran by the quenching effect on resonance light scattering and its application to water and vegetable samples

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A new method for the determination of carbofuran (CF) with DNA by a resonance light scattering (RLS) technique was developed. The intensity of RLS (IRLS) of DNA-HCl system was significantly quenched in presence of CF. A RLS peak at 315.6 nm was found, and the quenched intensity of RLS was proportional to the concentration of CF. The linear range of the calibration curve was ≍0.02-2.0 µg mL-1 and the detection limit (S/N = 3) 7 ng mL-1. The CF in river water, cucumbers and rice samples was determined. The recovery rates were in the range of 90.0-111.1, 95.0-106, 93.0-111.0%, respectively. The mechanism of the reaction between CF and DNA is also discussed.
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Keywords: DNA; carbofuran; food samples; quenching effect; resonance light-scattering method; river water

Document Type: Research Article

Affiliations: 1: Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, P.R. China 2: Yantai Research Institute, China Agricultural University, Yantai, P.R. China

Publication date: January 1, 2009

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