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Surface plasmon resonance-based DNA biosensor for arsenic trioxide detection

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Surface plasmon resonance-based biosensor has been fabricated using self-assembled monolayer (SAM) of -merceptoethanol (MCE) deposited on gold (Au) substrate. The double stranded calf thymus deoxyribonucleic acid (dsCT-DNA) has been immobilised onto MCE/Au electrode using N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) chemistry. This dsCT-DNA/MCE/Au bioelectrode has been characterised using cyclic voltammetry, scanning electron microscopy (SEM) and X-ray photoelectron spectrometry (XPS), respectively. The detection limit of arsenic trioxide has been estimated as 0.01 × 10-6 g mL-1.
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Keywords: DNA; arsenic trioxide; self-assembled monolayer; surface plasmon resonance; -merceptoethanol

Document Type: Research Article

Affiliations: Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, New Delhi, India

Publication date: January 1, 2009

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