This work presents a new sensor for ammonium determination based on interdigitated conductometric thin-film planar electrodes. It was fabricated by including nonactin in a plasticised poly(vinyl chloride) membrane deposited on the sensitive area of the transducer. The effects of pH, buffer concentration, the interferences of Na+, K+ and Ca2+ ions, the effect of ionic strength and of temperature were investigated. The linear working range and sensitivity of the biosensor were also determined. The sensitivity of the ammonium detection was around 6.2 µS µM-1 [image omitted] and the detection limit was 2 × 10-5 M. The biosensor exhibits the advantages of a simple fabrication procedure, good sensor-to-sensor reproducibility, wide dynamic range and good storage stability (12 weeks).
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