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Ammonia sensor with flavin-containing monooxygenase

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A biosensor for ammonia solution was developed with a flavin-containing monooxygenase-3 (FMO3). The biosensor consisted of a Clark-type dissolved oxygen electrode and an FMO3 immobilized membrane. In order to amplify the biosensor output, a substrate regeneration cycle obtained by coupling the monooxygenase with L-ascorbic acid (AsA) as the reducing reagent system, was applied. The AsA 10.0 mmol L–1 concentration was able to optimally amplify the sensor output 11 times greater. The FMO3 biosensor was used with AsA to measure ammonia solution from 3.20 to 14.29 mmol L–1 and from 0.09 to 21.7 mmol L–1 with 5.0 and 10.0 mmol L–1 AsA, respectively. The FMO biosensor also had a good reproducibility such as a 2.5% coefficient of variation in eight multiple measurements, and the output current was maintained over a few hours. The selectivity of the FMO biosensor being attributed to enzyme specificity was obtained for several chemical substances (trimethyl amine, methyl mercaptan, dimethyl sulphide, and so on).

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Keywords: Biosensor; Enzyme; Flavin-containing monooxygenase-3; L-Ascorbic acid; Reducing reagent system

Document Type: Research Article

Affiliations: 1: Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan 2: Tokai University, 1117 Kitakaname, Hiratsuka, Kanagawa 259-1292, Japan

Publication date: December 10, 2006

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