Skip to main content
padlock icon - secure page this page is secure

Application of an innovative matrix solid-phase dispersion-solid-phase extraction-liquid chromatography-tandem mass spectrometry analytical methodology to the study of the metabolism of the estrogenic mycotoxin zearalenone in rainbow trout liver and muscular tissue

Buy Article:

$61.00 + tax (Refund Policy)

The metabolic fate of the estrogenic mycotoxin zearalenone in rainbow trout is presently unknown. In this study, the tissue concentration of zearalenone and its principal metabolites (α-zearalenol and -zearalenol) was determined. A known amount of zearalenone was administered as a single bolus to ten fish, and the biological tissue concentration was determined at various times following administration. The analytes were extracted from liver and muscular tissue using an on-line matrix solid-phase dispersion-solid-phase extraction sample preparation protocol, and their concentration determined by HPLC-Turboionspray-tandem mass spectrometry. The results showed that zearalenone is mainly metabolized into α-zearalenol in both liver and muscular tissues. The maximum concentrations of each analyte found in liver were 76.1, 211.2 and 63.7 ng/g respectively for zearalenone, α-zearalenol and -zearalenol, while in muscular tissue they were 10.7, 8.2 and 6.5 ng/g. These values were reached after 2 h in liver tissue and 12 h in muscular tissue. Moreover the data obtained showed that the elimination rate in liver is quite fast since 48 h after the exposure less than 7% of the maximum concentration found is still present. In muscular tissue, however, about one-third of the maximum concentration found is still present after 48 h.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: HPLC-MS/MS; MSPD; Rainbow trout; Zearalenone

Document Type: Research Article

Publication date: November 1, 2004

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more