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Monitoring Environmental WasteWater Using A Piezoelectric Impedance Microbial Sensing Technique

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A new method was proposed for monitoring antibiotics and other pollutants based on the assessment of the growth of Staphylococcus aureus (S. aureus) with a piezoelectric impedance analysis technique. During the growth process of S. aureus, the motional resistance variation (ΔR1) increases and the frequency shift (Δf) of the piezoelectric quartz crystal (PQC) decreases correspondingly. The two responses can conveniently be used in process analysis. Under the given experimental conditions, a linear relationship between the resistance detection time (RDT) and logarithm value of the concentration of penicillin was obtained in the range of 0.01-10 µg/ml with a detection limit of 4 × 10-3 µg/ml. Other antibiotics and pollutants also have the similar linear relationships. The proposed method is simple, convenient and sensitive. Particularly, it does not need the immobilization of the microorganism. This method was used for monitoring of wastewater from a hospital.
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Keywords: Antibiotics; Environmental monitoring; Inhibition process; Piezoelectric impedance analysis; Wastewater

Document Type: Research Article

Affiliations: College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P.R. China

Publication date: January 1, 2002

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