Biological evaluation of redox stable cisplatin/Cu(II)-DNA adducts as potential anticancer agents
A series of non-enolizable β-diketonate-based copper(II) complexes with LCuCl2 [L = Knoevenagel condensates of curcumin (Salcimine) and methylacetoacetate (SalMaA)-based Schiff bases] chromospheres as functional models of chemotherapy drug cisplatin were investigated
for their covalent interaction with herring sperm DNA. The synthesis and structural characterization of 1a and 1b have been reported in our previous article. However, their DNA interactions and cytotoxicity properties were not studied. These analyses have been carried out mainly
through electrochemical techniques supplemented with spectral, relative viscosity, gel electrophoresis techniques, and AGS cancer cells using MTT assay. The cytotoxic activities of the ligand, curcumin-based copper complex, and cisplatin were tested against the AGS cancer cell line under similar
experimental conditions showing that the complex exhibited cancer cell inhibitory rate closer to cisplatin even at low concentration. This was also seen in the docking of the Cu-complex onto a rich guanine B-DNA decamer, where a Cu–N3(guanine) interaction instead of Pt-N7
as cisplatin is detected. The obtained results in this study prove that these complexes could be a promising substitute for cisplatin as a new family of non-platinum-based anticancer metallo-drugs after in vivo tests on animal models.
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Document Type: Research Article
Department of Materials Science, School of Chemistry, Madurai Kamaraj University, Madurai, India
Department of Chemistry, Sourastra College, Madurai, India
Department of Genetics, School of Biological Sciences, Madurai Kamaraj University, Madurai, India
Department of Inorganic Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, India
Department of Microbial Technology, School of Biological Sciences, Madurai Kamaraj University, Madurai, India
January 17, 2016