An oxopyrrolidine-based ligand [3-(4-chlorobenzylidine)-5-(5-amino-1,3,4-thiadiazol-2-yl)pyrrolidin-2-one] was synthesized by coupling pyroglutamic acid with p-chlorobenzaldehyde followed by cyclization with thiosemicarbazide in presence of oxyphosphoryl chloride. Copper, nickel,
and ruthenium complexes of the ligand were also synthesized. The electrolytic nature, with octahedral geometries for nickel and ruthenium complexes, was observed, while tetragonally distorted octahedral geometry for copper complex was proposed. The complexes were resistant to dissociation
and degradation in 5% DMSO solutions of PBS (pH 7.4). DNA binding constants (K
b) for ligand, copper, nickel, and ruthenium complexes were 1.15 × 105, 1.67 × 105, 1.87 × 105, and 1.007 × 106 M−1,
respectively, indicating quite strong binding with DNA. In silico studies also showed that the ligand interacted with DNA mainly through van der Waal’s forces. The docking energy of ligand−DNA adduct was –30.45 kJ M–1, suggesting a good
affinity of the reported ligand with DNA. Both the ligand and its complexes were less toxic to RBCs as compared to doxorubicin. All the compounds showed moderate anticancer activities on MCF-7 (wild type) breast cancer cell lines.
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In silico studies;
Document Type: Research Article
Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi, India
Department of Chemistry, College of Science, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia
Department of Biomedical Engineering, Chung Yuan Christian University, Chung Li, Taiwan
Publication date: June 18, 2014