Effect of polysaccharides from Ganoderma lucidum on streptozotocin-induced diabetic nephropathy in mice
The effects of Ganoderma lucidum polysaccharides (GL-PS) on renal complication in streptozotocin-induced diabetic mice have been investigated in the present study. C57BL/6J mice were made diabetic by injection of streptozotocin and GL-PS (125 and 250ÂmgÂkg–Â1) was administered for 8 weeks. Body weight was monitored every week. Serum glucose, creatinine (Cr), blood urea nitrogen (BUN), triglyceride (TG) and urinary albumin excretion (UAE) were measured after 8 weeks of treatment. Glomerular size and mesangial matrix index were assayed by morphometric analysis. Renal expression of transforming growth factor-β1 (TGF-β1) were determined by immunochemistry. Renal malondialdehyde (MDA) level and superoxide dismutase (SOD) activities were also evaluated. GL-PS was able to reduce the serum Cr and BUN levels and UAE compared with diabetic model mice in a dose-dependent manner. Increasing serum glucose and triglyceride levels in diabetic mice could also be lowered by GL-PS. Moreover, GL-PS had the capacity to improve the renal morphometric changes and oxidative stress state of diabetic mice. In summary, GL-PS can improve the metabolic abnormalities of diabetic mice and prevent or delay the progression of diabetic renal complications.
Keywords: Ganoderma lucidum; Diabetic nephropathy; Mouse; Polysaccharides
Document Type: Research Article
Affiliations: 1: Fuzhou Green Valley Institute of Bio-pharmaceutical Technology, Fuzhou, 350002, China 2: Department of Pharmacology, School of Basic Medical Sciences, Peking University Health Science Centre, Beijing, 100083, China 3: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100094, China
Publication date: 01 December 2006
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