Patensin-Induced Apoptosis is Accompanied by Decreased bcl-2 Expression and Telomerase Activity in HL-60 Cells
-1) for 3, 6, 12 and 24 h. Apoptosis and bcl-2 were determined by flow cytometry analysis. A polymerase-chain-reaction-based telomeric repeat amplification protocol assay was used to detect the telomerase activity. Patensin induced growth arrest and apoptotic cell death in HL-60 cells. The telomerase activity was inhibited in a time-dependent manner during the patensin-induced apoptosis of HL-60 cells, and the expression of bcl-2 was progressively down-regulated by patensin. Inhibition of the telomerase activity of HL-60 cells was closely related to the patensin-induced apoptosis. The present results indicate that inhibition in telomerase and reduced bcl-2 gene expression may play a role in the patensin-induced apoptosis of HL-60 cells.
Document Type: Research Article
Affiliations: 1: Department of Oncology Yijishan Hospital, Wannan Medical College Wuhu 241001 Anhui China 2: Department of Phytochemistry China Pharmaceutical University 210009 Nanjing China 3: Department of Pharmacology China Pharmaceutical University 210009 Nanjing China
Publication date: June 1, 2004