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Patensin-Induced Apoptosis is Accompanied by Decreased bcl-2 Expression and Telomerase Activity in HL-60 Cells

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The present study aimed to investigate the effect of telomerase activity in patensin-induced apoptosis and the regulation of B cell leukemia/lymphoma 2 (bcl-2) gene expression in human leukemia HL-60 cells. Apoptosis of HL-60 cells was induced by patensin (100 μmol L
-1
) for 3, 6, 12 and 24 h. Apoptosis and bcl-2 were determined by flow cytometry analysis. A polymerase-chain-reaction-based telomeric repeat amplification protocol assay was used to detect the telomerase activity. Patensin induced growth arrest and apoptotic cell death in HL-60 cells. The telomerase activity was inhibited in a time-dependent manner during the patensin-induced apoptosis of HL-60 cells, and the expression of bcl-2 was progressively down-regulated by patensin. Inhibition of the telomerase activity of HL-60 cells was closely related to the patensin-induced apoptosis. The present results indicate that inhibition in telomerase and reduced bcl-2 gene expression may play a role in the patensin-induced apoptosis of HL-60 cells.
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Keywords: Apoptosis; Bcl-2 gene; HL-60 cell; Patensin; Telomerase

Document Type: Research Article

Affiliations: 1: Department of Oncology Yijishan Hospital, Wannan Medical College Wuhu 241001 Anhui China 2: Department of Phytochemistry China Pharmaceutical University 210009 Nanjing China 3: Department of Pharmacology China Pharmaceutical University 210009 Nanjing China

Publication date: June 1, 2004

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