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Designing a New Recombinant Indigenous Klebsiella oxytoca ISA4 by Cloning of dsz Genes

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A bacterium designated as a Klebsiella oxytoca ISA4 was isolated from oil-contaminated soil; it not only is unable to catabolize dibenzothiophene as a sole source of carbon and energy but also produces biosurfactant. In order to enhance the efficiency of biodesulfurization, the dszABC genes from Rhodococcus erythropolis IGTS8 were amplified by specific primers. PCR ampilicon of 3.8 kb was obtained and cloned using pVLT31 vector in native K. oxytoca ISA4. Further analysis by Gibbs assay and gas chromatograph revealed that recombinant K. oxytoca ISA4 has the highest desulfurization ability (48%) in comparison with R. erythropolis IGTS8 (42%) and Pseudomonas aeruginosa pTSOX4 (46%).
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Keywords: Klebsiella oxytoca; Rhodococcus erythropolis IGTS8; biodesulfurization; dibenzothiophene; dszABC

Document Type: Research Article

Affiliations: 1: Faculty of Biological Science, Shahid Beheshti University, Tehran, Iran 2: Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

Publication date: October 2, 2015

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