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M olecular (PCR) differentiation of Giardia muris and Giardia intestinalis

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Using random amplification of polymorphic DNA (RAPD) a band present with G. muris but not G. intestinalis DNA was detected. This band was cloned and sequenced and this facilitated the selection of a pair of primers specific for G. muris. Since G. muris does not infect humans, the use of these primers in conjunction with primers specific for G. intestinalis should enable water to be determined as safe or unsafe for human consumption with respect to the species of Giardia.
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Keywords: G. INTESTINALIS; G. MURIS; GIARDIA; SPECIES DIFFERENTIATION

Document Type: Research Article

Publication date: March 1, 1997

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