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Home / Food and Agricultural Immunology, Volume 30, Number 1

Open Access Simple and sensitive detection of triazophos pesticide by using quantum dots nanobeads based on immunoassay

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Authors: Liao, Yun 1 ; Cui, Xueyan 2 ; Chen, Ge 2 ; Wang, Yuanshang 2 ; Qin, Guoxin 3 ; Li, Mingjie 2 ; Zhang, Xiuyuan 2 ; Zhang, Yudan 2 ; Zhang, Chan 2 ; Du, Pengfei 2 ; Yan, Feiyan 3 ; El-Aty, A. M. Abd 4 ; Wang, Jing 2 ; Zhang, Chenghui 1 ; Jin, Maojun 2 ; 

Source: Food and Agricultural Immunology, Volume 30, Number 1, 1 January 2019, pp. 522-532(11)

Publisher: Taylor and Francis Ltd

DOI: https://doi.org/10.1080/09540105.2019.1597022

This article is Open Access under the terms of the Creative Commons CC BY-NC-ND licence.

A rapid, ultra-sensitive and simple fluorescence immunoassay (FLISA) on CdSe/ZnS Quantum dots (QDs) for recognition pesticide triazophos have been established. In this assay, the immunoassay uses (CdSe/ZnS) Quantum dots (QDs) as a probe that carries monoclonal antibody (mAb). The free triazophos and OVA-haptens were competed for binding the mAb on the surface of the QDs. Therefore, the concentration of TRIAZ can get by calculation of the detection of fluorescence value. The assay has a linear response in the 10 ng L−1–25 μg L−1 TRIAZ concentration range, and limit of detection (IC10) was 0.508 ng L−1. The recovery rate in the case of spiked samples ranges from 82.6% to 96.6%, and the RSD is <20%. FLISA can accurately and sensitively screen out triazophos in representative actual samples that contain trace levels of pesticides, it can play a prominent part in agricultural product and environmental detection.

Keywords: Immunoassay; antibody; fluorescence; quantum dots

Document Type: Research Article

Affiliations: 1: College of Food Science and Technology, Hainan University, Haikou, People’s Republic of China 2: Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Science, Beijing, People’s Republic of China 3: Agro-products Quality Safety and Testing Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, People’s Republic of China 4: Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt

Publication date: 01 January 2019

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