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In vitro synthesis of a type A trichothecenes complete antigen from T-2 toxin

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We report the artificial synthesis from T-2 toxin of a type A trichothecenes complete antigen. First, 3-Ac-T-2 was made from T-2 following acetylation. Then 3-Ac-NEOS as a T-2 skeleton structure was synthesized by enzymolysis and identified by liquid chromatography-tandem mass spectrometry and nuclear magnetic resonance. Next, a hapten (3-Ac-NEOS-HS) was formed by modifying the C8 position of 3-Ac-NEOS using succinic anhydride method. 3-Ac-NEOS-HS-BSA/-OVA were prepared by conjugating hapten with bovine serum albumin (BSA) or ovalbumin (OVA) by carbodiimide method and identified by UV spectroscopy and sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Results showed that conjugation ratio of 3-Ac-NEOS-HS to BSA was 8.76: 1 and OVA 7.24: 1, indicating 3-Ac-NEOS-HS-BSA as a complete antigen was better. Next we used it to immunize rabbits and obtained a 1:64,000 antibody titre. In conclusion, a type A trichothecenes complete antigen was successfully synthesized, which was the foundation for antibody preparation and enzyme-linked immunosorbent assay kit development for all type A trichothecenes (parent + modified/masked type A trichothecenes).
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Keywords: 3-Ac-NEOS; T-2 toxin; Type A trichothecenes; complete antigen; skeleton

Document Type: Research Article

Affiliations: 1: College of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang, 524088, China 2: College of Environment and Resources, Dalian Nationalities University, Dalian, 116600, China 3: Food Safety Key Laboratory of Liaoning Province, Bohai University, Jinzhou, 121013, China 4: Centre for Food Research and Innovation, Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln, 7647, Canterbury, New Zealand

Publication date: November 1, 2016

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