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Development of an immunochromatographic assay for hexestrol and diethylstilbestrol residues in milk

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Hexestrol (HES) and Diethylstilbestrol (DES) are synthetic hormones, which have been found abuse use in animal-origin food production. We developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immune-chromatographic strip to detect these compounds based on monoclonal antibody. Under optimized conditions, the half-inhibition concentration (IC50) values of ic-ELISA were 0.15 µgL−1 and 0.23 µgL−1 for HES and DES, respectively. Spiked milk samples were analyzed. The recovery of both synthetic hormones in the milk samples was 60.48–102.19% (HES) and 89.34–100.16% (DES). In immune-chromatographic assay, the visual cutoff values at 0.5 and 1.0 µgL−1 respectively for HES and DES, which allow us to detect milk samples of a concentration low to 10 µgL−1 for HES and 15 µgL−1 for DES.
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Keywords: Hexestrol; diethylstilbestrol; ic-ELISA; immunochromatographic assay; milk; monoclonal antibody

Document Type: Research Article

Affiliations: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, People’s Republic of China

Publication date: November 1, 2016

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