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Preparation, immunological characterization and polyclonal antibody development for recombinant epitope tandem derived from bovine β-lactoglobulin

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Bovine β-lactoglobulin (BLG) is one of the major allergens in cow’s milk. Epitopes play a key role in food allergy, while most of the antibodies developed do not specifically recognize the IgE-binding epitopes. Here, a tandem derived from human IgE linear epitopes of BLG (tBLG) was designed according to the principles of epitope-based vaccine methodology, expressed in Escherichia coli, and a polyclonal antibody (pAb-tBLG) was developed. The recombinant tBLG protein preserved the antigenicity and potential allergenicity of epitopes. The pAb-tBLG recognized all the IgE epitopes of tBLG, and showed a high specificity, but it had weak cross-reactivity with caseins. We concluded that pAb-tBLG can detect of BLG and may be useful for identifying allergenic residues in dairy products.
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Keywords: ELISA; Milk allergy; epitope tandem; polyclonal antibody; β-lactoglobulin

Document Type: Research Article

Affiliations: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China

Publication date: November 1, 2016

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