@article {Peng:2016:0954-0105:449,
title = "Rapid enzyme-linked immunosorbent assay and immunochromatographic strip for detecting ribavirin in chicken muscles",
journal = "Food and Agricultural Immunology",
parent_itemid = "infobike://tandf/cfai",
publishercode ="tandf",
year = "2016",
volume = "27",
number = "4",
publication date ="2016-07-03T00:00:00",
pages = "449-459",
itemtype = "ARTICLE",
issn = "0954-0105",
eissn = "1465-3443",
url = "https://www.ingentaconnect.com/content/tandf/cfai/2016/00000027/00000004/art00002",
doi = "doi:10.1080/09540105.2015.1104657",
keyword = "immunochromatographic strip, chicken tissue, ic-ELISA, ribavirin",
author = "Peng, Shuang and Song, Shanshan and Liu, Liqiang and Kuang, Hua and Xu, Chuanlai",
abstract = "The use of ribavirin (RBV) as an antiviral drug for livestock has been prohibited in China, the USA, and many other countries. In this study, we developed a rapid and sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a gold nanoparticle immunochromatographic
(ICA) strip test for detecting RBV in chicken muscles. Under the optimum assay conditions, where the assay employed phosphate-buffered saline at pH 7.4, no acetonitrile, and an ionic strength of 0.8%, the quantitative working range was 1.4326.47ng/ml with an IC50
of 6.15ng/ml. The recovery rate for RBV in real samples ranged from 82.1% to 112.3%. The immunochromatographic test strip method had a visual cutoff value of 50g/kg. Given their high recovery rates and good sensitivity, the proposed ic-ELISA and ICA methods could be useful
for the RBV analysis in chicken tissue samples.",
}