Determination of oxolinic acid residues in the muscle tissue of olive flounder (Paralichthysolivaceus) by a lateral flow immunoassay
This study was designed to determine oxolinic acid (OA) residues in the muscle of olive flounder (Paralichthysolivaceus) by a lateral flow immunoassay (LFIA) and ultra-performance liquid chromatography coupled with fluorescence detection (UPLC–FLD). After 25 cultured olive
flounder were treated by dipping in water dosed with OA (50 mg/L water for 1 h), muscle tissues were sampled and prepared for the detection of OA residues. The visual detection limit of the test strip for OA determined by the C/T ratio of 1 was 28 µg/kg of muscle.
The detection limit of OA based on the standard curve was 4 and 1.6 µg/kg of muscle using LFIA and UPLC–FLD, respectively, and recoveries of all spiked samples by LFIA and UPLC–FLD were >96%. LFIA and UPLC–FLD showed good correlation (R
2 > .9997)
for a series of samples collected over 5 days post treatment. The LFIA is applicable to the detection of OA in the muscle tissue of farmed fish, and is an effective and economical method that will enable high-throughput screening.
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lateral flow immunoassay;
ultra-performance liquid chromatography
Document Type: Research Article
Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University, Chinju, Republic of Korea
Food and Drug Analysis Section, Chungcheongbuk-do Institute of Health and Environment, Cheongwon, Korea
Department of Bioengineering, Graduate School of Science and Engineering, Bioengineering Division, Hacettepe University, Ankara, Turkey
Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University, Busan, Republic of Korea
May 3, 2016
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