Skip to main content
padlock icon - secure page this page is secure

Determination of oxolinic acid residues in the muscle tissue of olive flounder (Paralichthysolivaceus) by a lateral flow immunoassay

Buy Article:

$63.00 + tax (Refund Policy)

This study was designed to determine oxolinic acid (OA) residues in the muscle of olive flounder (Paralichthysolivaceus) by a lateral flow immunoassay (LFIA) and ultra-performance liquid chromatography coupled with fluorescence detection (UPLC–FLD). After 25 cultured olive flounder were treated by dipping in water dosed with OA (50 mg/L water for 1 h), muscle tissues were sampled and prepared for the detection of OA residues. The visual detection limit of the test strip for OA determined by the C/T ratio of 1 was 28 µg/kg of muscle. The detection limit of OA based on the standard curve was 4 and 1.6 µg/kg of muscle using LFIA and UPLC–FLD, respectively, and recoveries of all spiked samples by LFIA and UPLC–FLD were >96%. LFIA and UPLC–FLD showed good correlation (R 2 > .9997) for a series of samples collected over 5 days post treatment. The LFIA is applicable to the detection of OA in the muscle tissue of farmed fish, and is an effective and economical method that will enable high-throughput screening.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: lateral flow immunoassay; muscle residues; olive flounder; oxolinic acid; ultra-performance liquid chromatography

Document Type: Research Article

Affiliations: 1: Research Institute of Life Sciences, College of Veterinary Medicine, Gyeongsang National University, Chinju, Republic of Korea 2: Food and Drug Analysis Section, Chungcheongbuk-do Institute of Health and Environment, Cheongwon, Korea 3: Department of Bioengineering, Graduate School of Science and Engineering, Bioengineering Division, Hacettepe University, Ankara, Turkey 4: Department of Medical Humanities and Social Medicine, College of Medicine, Kosin University, Busan, Republic of Korea

Publication date: May 3, 2016

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more