Mechanistic study of signalling pathways in intestinal B lymphocytes using eicosapentaenoic and docosahexaenoic acid in broiler chickens
The present study was conducted to investigate the effects of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), and a combination of both at a ratio of 2:1 on the signalling pathways in lipopolysaccharide (LPS)-stimulated intestinal B lymphocytes from broiler chickens. The results showed that these treatments reduced the production of prostaglandin E2 (PGE2), cyclooxygenase 2 (COX-2), and phospholipase C (PLC) and increased the concentration of inositol triphosphate (IP3) significantly compared with the control group (P < .05). Compared with EPA or DHA alone, the combination of both (2:1) reduced the production PGE2, COX-2, and PLC and increased the concentration of IP3 (P < .05). Therefore, EPA and DHA suppressed the LPS-stimulated proliferation of B lymphocytes by interfering with phosphatidylinositol signalling and the second messenger pathways.
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Document Type: Research Article
Affiliations: College of Animal Science & Technology, Northwest A&F University, Yang Ling, Shaan'xi, People's Republic of China
Publication date: May 3, 2016