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Prolamins of maize and wheat differentially affect intestinal cells both in biopsies of celiac patients and CACO-2 cell line

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We study the effect of maize zeins on cellular immune response as compared to that of wheat gliadins for exacerbating celiac disease due to a hypothetical similar response in some patients. In vitro activation of celiac duodenal mucosa with gliadin or zein immunoreactive peptides and pro-inflammatory response of CACO-2 cells to digested gliadin or zein fractions were evaluated as indicators of adaptive and innate response, respectively. In 3/5 biopsies, zein increased production of IFN-γ, whereas gliadin has done it in all the patient biopsies. In CACO-2 cells, two zein fractions (3–5 and 1–3 kDa), similar to gliadin fractions, stimulated the production of IL-8, p38 MAPK, COX2, and release of ZO-1 as compared to medium alone. The 3–5 kDa zein fraction increased permeability in cell monolayers, although less than gliadin. Zein peptides are immunogenic for some patients and induce a similar innate response, but to a lesser extent than gliadin peptides.
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Keywords: celiac disease; cell immune response; maize prolamins; zeins

Document Type: Research Article

Affiliations: 1: Departamento de Nutrición y Metabolismo, Centro de Investigación en Alimentación y Desarrollo, A.C. Carr. La Victoria, km 0.6, P.O. Box 1735, Hermosillo, Sonora, 83304, México 2: Coordinación de Ciencia de los Alimentos, Centro de Investigación en Alimentación y Desarrollo, A.C. Carr. La Victoria, km 0.6, P.O. Box 1735, Hermosillo, Sonora, 83304, México 3: Unidad Académica de Nutrición y Gastronomía, Universidad Autónoma de Sinaloa, Culiacán, Sinaloa, 80019, México

Publication date: March 3, 2016

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