@article {Wang:2016:0954-0105:230,
title = "The matrix interference to the immunoassay of food samples: The effect of some proteins in aquatic products",
journal = "Food and Agricultural Immunology",
parent_itemid = "infobike://tandf/cfai",
publishercode ="tandf",
year = "2016",
volume = "27",
number = "2",
publication date ="2016-03-03T00:00:00",
pages = "230-241",
itemtype = "ARTICLE",
issn = "0954-0105",
eissn = "1465-3443",
url = "https://www.ingentaconnect.com/content/tandf/cfai/2016/00000027/00000002/art00007",
doi = "doi:10.1080/09540105.2015.1086317",
keyword = "aquatic products, matrix interference, protein, immunoassay",
author = "Wang, Xiudan and Lin, Hong and Cao, Limin and Zhang, Xue and Sui, Jianxin",
abstract = "The elimination of matrix interference is now a task for further development and application of immunoassays. Here the effect of proteins in aquatic samples on immunoassay was investigated by western blotting and competitive indirect enzyme-labeled immunosorbent assay (ci-ELISA). Some
proteins in flatfishes interacted with different antibodies, resulting in significant loss of accuracy of the immunoassays. Moreover, these matrix proteins prefer to interact with enzyme-labeled antibodies than label-free ones. Such a matrix proteinimmunoglobulin interaction was validated
with different fish and shrimp samples and similar results were observed, which demonstrated a universal significance of the protein-induced matrix interference to immunoassays. Besides, the strategies to prevent such interference were preliminarily discussed.",
}