An effective biotin–streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) to rapidly detect metolcarb (MTMC) residues is reported. Nonspecific adsorption was minimized by using 0.5% skimmed milk powder as a blocking buffer and 0.5% bovine serum albumin/phosphate-buffered
saline (PBS) as a buffer for streptavidin–horse radish peroxidase conjugates. The established method is four times sensitive than direct competitive enzyme-linked immunosorbent assay, with the IC50 of 10.0 ng mL−1. The samples were prepared from
mustard, cucumber and mushroom with simple extraction and dilution methods, including use of PBS without concentration or cleanup steps. The samples prepared from spinach and shiitake were quantified after 2-fold methanol extraction and 20-fold dilution with 2.0% fish glutin/PBS. Good accuracy
and precision were obtained with mean recoveries between 80.0% and 95.6%, and mean coefficients of variation below 12.1%. A good correlation (R
2 = 0.9931) between the BA-ELISA and high-performance liquid chromatography was observed for MTMC analysis in different
samples. This method could be potentially useful for high-throughput food inspection.
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biotin–streptavidin amplified ELISA;
Document Type: Research Article
Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, People's Republic of China
Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, People's Republic of China
March 3, 2016
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