Skip to main content
padlock icon - secure page this page is secure

Determination of metolcarb residues by a biotin–streptavidin-amplified enzyme-linked immunosorbent assay in vegetables and edible fungus

Buy Article:

$63.00 + tax (Refund Policy)

An effective biotin–streptavidin-amplified enzyme-linked immunosorbent assay (BA-ELISA) to rapidly detect metolcarb (MTMC) residues is reported. Nonspecific adsorption was minimized by using 0.5% skimmed milk powder as a blocking buffer and 0.5% bovine serum albumin/phosphate-buffered saline (PBS) as a buffer for streptavidin–horse radish peroxidase conjugates. The established method is four times sensitive than direct competitive enzyme-linked immunosorbent assay, with the IC50 of 10.0 ng mL−1. The samples were prepared from mustard, cucumber and mushroom with simple extraction and dilution methods, including use of PBS without concentration or cleanup steps. The samples prepared from spinach and shiitake were quantified after 2-fold methanol extraction and 20-fold dilution with 2.0% fish glutin/PBS. Good accuracy and precision were obtained with mean recoveries between 80.0% and 95.6%, and mean coefficients of variation below 12.1%. A good correlation (R 2 = 0.9931) between the BA-ELISA and high-performance liquid chromatography was observed for MTMC analysis in different samples. This method could be potentially useful for high-throughput food inspection.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: HPLC; biotin–streptavidin amplified ELISA; nonspecific adsorption; sample pre-treatment

Document Type: Research Article

Affiliations: 1: Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University, People's Republic of China 2: Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, People's Republic of China

Publication date: March 3, 2016

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more