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Development of sandwich ELISA for quantification of the orange allergen profilin (Cit s 2)

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Oranges (Citrus sinensis) can provoke an allergic reaction in some individuals. The concentration of profilin (Cit s 2) – the major allergen in oranges – is unknown, but this knowledge could be useful for preventing the development of allergies. The aim of this study was to quantify the amount of Cit s 2 in Navel oranges and in different varieties of citrus fruit. A sandwich enzyme-linked immunosorbent assay (ELISA) was developed using both monoclonal and polyclonal antibodies. Extracts of Navel oranges and 12 citrus fruits were tested using this ELISA. Cit s 2 levels in the Navel orange pulp and peel were 1.81 ± 0.74 and 1.19 ± 0.87 µg/g, respectively, with accumulation detected primarily in the pulp. These findings may help in the production of low-risk processed oranges for orange allergy sufferers through selection of orange varieties and parts with low profilin content during food processing.
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Keywords: Citrus sinensis; food allergy; orange allergen; profilin; sandwich ELISA

Document Type: Research Article

Affiliations: 1: Division of Food Chemistry, Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka, 537-0025, Japan 2: Division of Bacteriology, Osaka Prefectural Institute of Public Health, 1-3-69 Nakamichi, Higashinari-ku, Osaka, 537-0025, Japan

Publication date: January 2, 2016

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