Provider: Ingenta Connect Database: Ingenta Connect Content: application/x-research-info-systems TY - ABST AU - Hsu AU - Hsu AU - Lin TI - Yam storage protein dioscorins modulate cytokine gene expression in BALB/c and C57BL/6 lymphocytes JO - Food and Agricultural Immunology PY - 2015-11-02T00:00:00/// VL - 26 IS - 6 SP - 909 EP - 923 KW - cytokine array KW - polymyxin B KW - traditional herbal medicine KW - dioscorin N2 - This study aimed to evaluate the effects of dioscorins isolated from 2 yam species, Dioscorea alata (Da-dioscorins) and Dioscorea japonica (Dj-dioscorins), on mouse immune activities. Results from cytokine array indicated that Dj-dioscorins increased the production of tumor necrosis factor (TNF)-α, interleukin (IL)-2, IL-3, IL-6, IL-10, IL-12p40, IL-13, monocyte chemoattractant protein (MCP)-1, regulated on activation, normal T cell expressed and secreted (RANTES), and granulocyte colony-stimulating factor (GCSF) in the spleen cells of BALB/c mice. In RAW264.7 macrophage, Da-dioscorins upregulated the expression of TNF-α, IL-1β, IL-6, IL-10, RANTES, MCP-1, and GCSF genes to a greater extent than Dj-dioscorins did. TNF-α, IL-1β, IL-6, IL-10, and RANTES gene expression was higher in spleen cells than in bone marrow and thymus cells in Da-dioscorin- or Dj-dioscorin-treated BALB/c and C57BL/6. Overall, our results suggest that dioscorins exert distinct immunomodulatory effects on mouse immune cells, and that different mouse strains respond differently to dioscorins. UR - https://www.ingentaconnect.com/content/tandf/cfai/2015/00000026/00000006/art00013 M3 - doi:10.1080/09540105.2015.1048787 UR - https://doi.org/10.1080/09540105.2015.1048787 ER -