Skip to main content
padlock icon - secure page this page is secure

Preparation of a polyclonal antibody against the cadmium–DTPA complex and its application for determination of cadmium

Buy Article:

$63.00 + tax (Refund Policy)

Cadmium (Cd) is considered one of the most hazardous heavy metals. Therefore, it is important to detect Cd. A polyclonal antibody that recognizes Cd–ethylenediamine-N,N,N′,N′-tetraacetic acid complex was prepared via the injection of BALB/c mice with Cd–2-(4-aminobenzyl)-diethylene triamine pentaacetic acid–keyhole limpet hemocyanin. A simple, inexpensive, and reliable indirect competitive enzyme-linked immunosorbent assay (ELISA) based on the polyclonal antibody was developed for determination of Cd. The assay was specific to Cd with an IC50 value of 2.042 μg mL−1 and the detection limit of 0.0135 μg mL−1. Cross-reactivities (CRs) with Cu2+, Pb2+, Zn2+, Mg2+, Ca2+, Fe2+, and Ni2+ were below 0.001% except for Hg2+ with a CR less than 6%. The recoveries from deionized water were from 92.2% to 116.0%. Results indicated that the ELISA based on the polyclonal antibody would be very promising analytical tools for rapid and sensitive determination of metal ions in the environment.
No Reference information available - sign in for access.
No Citation information available - sign in for access.
No Supplementary Data.
No Article Media
No Metrics

Keywords: cadmium; determination; enzyme-linked immunosorbent assay; polyclonal antibody

Document Type: Research Article

Affiliations: School of Agriculture and Biology & Bor S. Luh Food Safety Research Center, Shanghai Jiaotong University, Shanghai, P.R. China

Publication date: November 2, 2015

More about this publication?
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more