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Preparation of a polyclonal antibody against the cadmium–DTPA complex and its application for determination of cadmium

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Cadmium (Cd) is considered one of the most hazardous heavy metals. Therefore, it is important to detect Cd. A polyclonal antibody that recognizes Cd–ethylenediamine-N,N,N′,N′-tetraacetic acid complex was prepared via the injection of BALB/c mice with Cd–2-(4-aminobenzyl)-diethylene triamine pentaacetic acid–keyhole limpet hemocyanin. A simple, inexpensive, and reliable indirect competitive enzyme-linked immunosorbent assay (ELISA) based on the polyclonal antibody was developed for determination of Cd. The assay was specific to Cd with an IC50 value of 2.042 μg mL−1 and the detection limit of 0.0135 μg mL−1. Cross-reactivities (CRs) with Cu2+, Pb2+, Zn2+, Mg2+, Ca2+, Fe2+, and Ni2+ were below 0.001% except for Hg2+ with a CR less than 6%. The recoveries from deionized water were from 92.2% to 116.0%. Results indicated that the ELISA based on the polyclonal antibody would be very promising analytical tools for rapid and sensitive determination of metal ions in the environment.
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Keywords: cadmium; determination; enzyme-linked immunosorbent assay; polyclonal antibody

Document Type: Research Article

Affiliations: School of Agriculture and Biology & Bor S. Luh Food Safety Research Center, Shanghai Jiaotong University, Shanghai, P.R. China

Publication date: November 2, 2015

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