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Novel F0F1-ATPase molecular motor biosensors for rapid detection of foodborne pathogenic bacteria

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F0F1-ATPase molecular motor biosensors were constructed by connecting specific probes to F0F1-ATPase’s ε-subunit through the avidin–biotin system. The test sample and negative sample were combined with the biosensors, respectively, and the DNA of the pathogenic bacteria can be tested by the comparison of the production of catalytically synthesized ATP after 10 min. The amount of synthesized ATP can be indirectly determined by the fluorescence intensity of luciferase–luciferin testing reagent. Through the comparison of the detection results of actual samples using three different methods, it was found that the method described in this study showed excellent agreement with the traditional detection method and polymerase chain reaction (PCR) detection method. The results also indicated that the F0F1-ATPase molecular motor biosensor could specifically detect bacterial DNA at a very low concentration level, and it would be a convenient, quick, and promising tool for pathogens detection.
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Keywords: F0F1-ATPase molecular motor; foodborne pathogenic bacteria; rapid detection

Document Type: Research Article

Affiliations: 1: Beijing Entry–Exit Inspection and Quarantine Bureau, Beijing, 100026, China 2: Huai'an Entry–Exit Inspection and Quarantine Bureau, Huai’an, 223001, China 3: Laboratory of Guangdong International Travel Health Care Center, Guangzhou, 510635, China

Publication date: September 3, 2015

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